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Part:BBa_K3814038
Designed by: Simon Tang Group: iGEM21_Sydney_Australia (2021-10-01)
fliK upstream homology region
This 50bp section of DNA is from the fliK gene that is going to be used as a homology arm for recombineering. As part of USYD 2021's project, we have developed a novel recombineering strategy (Babushka Blocks) to insert large amounts of linear DNA into the E. coli chromosome.
This is a region of the fliK gene, and gene clusters will be recombineered into it in E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |